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easysep negative selection human progenitor cell enrichment cocktail (stemcell technologies)  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc easysep negative selection human progenitor cell enrichment cocktail (stemcell technologies)
    Targeting Polθ + PARP and Polθ + RAD52 induced dual synthetic lethality against HR-deficient leukemia cells. A, Western blot analysis showing the expression of indicated proteins in the nuclear fractions of Nalm6(RAD54+/+) and Nalm6(RAD54−/−) isogenic cell lines. B, HR activity in Nalm6(RAD54+/+) and Nalm6(RAD54−/−) cells: results represent mean % SD of GFP+ cells in RFP+ cells. Nalm6(RAD54+/+) and Nalm6(RAD54−/−) cells were treated for 72 hours (C) and 12 hours (D) with 25 mmol/L Polθi ART558, 2.5 nmol/L PARPi talazoparib, 10 mmol/L RAD52i 6-hydroxy-DL-dopa and the indicated combinations. Results show mean number SD of colony numbers (C) and mean % of tail DNA SD (D). E, Western blot analysis showing the expression of indicated proteins in the nuclear and cytosolic fractions of HEL cells with tet-inducible IDH1wt or IDH1(R132H) mutant. F, HR activity in HEL cells expressing IDH1wt or IDH1(R132H) mutant: results represent mean % SD of GFP+ cells in RFP+ cells. HEL cells expressing IDH1wt or IDH1(R132H) mutant were treated for 72 hours (G) and 12 hours (H) with 25 mmol/L Polθi ART558, 1 mmol/L PARPi olaparib, 2.5 mmol/L RAD52i 6-hydroxy-DL-dopa and the indicated combinations. Results show mean % SD of colony numbers when compared with untreated cells (G) and mean % of tail DNA SD (H). I, HR activity in <t>Lin-CD34+</t> cells from HR-proficient and HR-deficient AMLs (n = 3 of each). Results represent mean % SD of GFP+ cells in RFP+ cells. J, <t>Lin-CD34+</t> cells from HR-proficient and HR-deficient AMLs (n = 3 of each) were treated with 25 mmol/L Polθi ART558, 1.25 mmol/L PARPi olaparib, 2.5 mmol/L RAD52i 6-hydroxy-DL–dopa and the indicated combinations. Results represent mean % SD of colonies in comparison with untreated cells. K, Survival curves of the AML mice treated with vehicle (C), Polθi RP6685, PARPi olaparib, and the combination. (B–D and F–J) Statistical significance when compared with: # another group, + control, and * corresponding individual treatments. K, ***, P < 0.001 in comparison to other groups.
    Easysep Negative Selection Human Progenitor Cell Enrichment Cocktail (Stemcell Technologies), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+negative+selection+human+progenitor+cell+enrichment+cocktail+(stemcell+technologies)/easysep+negative+selection+human+progenitor+cell+enrichment+cocktail/pmc10654933-64-25-30
    Average 90 stars, based on 1 article reviews
    easysep negative selection human progenitor cell enrichment cocktail (stemcell technologies) - by Bioz Stars, 2026-09
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    1) Product Images from "Simultaneous Targeting of DNA Polymerase Theta and PARP1 or RAD52 Triggers Dual Synthetic Lethality in Homologous Recombination–Deficient Leukemia Cells"

    Article Title: Simultaneous Targeting of DNA Polymerase Theta and PARP1 or RAD52 Triggers Dual Synthetic Lethality in Homologous Recombination–Deficient Leukemia Cells

    Journal: Molecular cancer research : MCR

    doi: 10.1158/1541-7786.MCR-22-1035

    Targeting Polθ + PARP and Polθ + RAD52 induced dual synthetic lethality against HR-deficient leukemia cells. A, Western blot analysis showing the expression of indicated proteins in the nuclear fractions of Nalm6(RAD54+/+) and Nalm6(RAD54−/−) isogenic cell lines. B, HR activity in Nalm6(RAD54+/+) and Nalm6(RAD54−/−) cells: results represent mean % SD of GFP+ cells in RFP+ cells. Nalm6(RAD54+/+) and Nalm6(RAD54−/−) cells were treated for 72 hours (C) and 12 hours (D) with 25 mmol/L Polθi ART558, 2.5 nmol/L PARPi talazoparib, 10 mmol/L RAD52i 6-hydroxy-DL-dopa and the indicated combinations. Results show mean number SD of colony numbers (C) and mean % of tail DNA SD (D). E, Western blot analysis showing the expression of indicated proteins in the nuclear and cytosolic fractions of HEL cells with tet-inducible IDH1wt or IDH1(R132H) mutant. F, HR activity in HEL cells expressing IDH1wt or IDH1(R132H) mutant: results represent mean % SD of GFP+ cells in RFP+ cells. HEL cells expressing IDH1wt or IDH1(R132H) mutant were treated for 72 hours (G) and 12 hours (H) with 25 mmol/L Polθi ART558, 1 mmol/L PARPi olaparib, 2.5 mmol/L RAD52i 6-hydroxy-DL-dopa and the indicated combinations. Results show mean % SD of colony numbers when compared with untreated cells (G) and mean % of tail DNA SD (H). I, HR activity in Lin-CD34+ cells from HR-proficient and HR-deficient AMLs (n = 3 of each). Results represent mean % SD of GFP+ cells in RFP+ cells. J, Lin-CD34+ cells from HR-proficient and HR-deficient AMLs (n = 3 of each) were treated with 25 mmol/L Polθi ART558, 1.25 mmol/L PARPi olaparib, 2.5 mmol/L RAD52i 6-hydroxy-DL–dopa and the indicated combinations. Results represent mean % SD of colonies in comparison with untreated cells. K, Survival curves of the AML mice treated with vehicle (C), Polθi RP6685, PARPi olaparib, and the combination. (B–D and F–J) Statistical significance when compared with: # another group, + control, and * corresponding individual treatments. K, ***, P < 0.001 in comparison to other groups.
    Figure Legend Snippet: Targeting Polθ + PARP and Polθ + RAD52 induced dual synthetic lethality against HR-deficient leukemia cells. A, Western blot analysis showing the expression of indicated proteins in the nuclear fractions of Nalm6(RAD54+/+) and Nalm6(RAD54−/−) isogenic cell lines. B, HR activity in Nalm6(RAD54+/+) and Nalm6(RAD54−/−) cells: results represent mean % SD of GFP+ cells in RFP+ cells. Nalm6(RAD54+/+) and Nalm6(RAD54−/−) cells were treated for 72 hours (C) and 12 hours (D) with 25 mmol/L Polθi ART558, 2.5 nmol/L PARPi talazoparib, 10 mmol/L RAD52i 6-hydroxy-DL-dopa and the indicated combinations. Results show mean number SD of colony numbers (C) and mean % of tail DNA SD (D). E, Western blot analysis showing the expression of indicated proteins in the nuclear and cytosolic fractions of HEL cells with tet-inducible IDH1wt or IDH1(R132H) mutant. F, HR activity in HEL cells expressing IDH1wt or IDH1(R132H) mutant: results represent mean % SD of GFP+ cells in RFP+ cells. HEL cells expressing IDH1wt or IDH1(R132H) mutant were treated for 72 hours (G) and 12 hours (H) with 25 mmol/L Polθi ART558, 1 mmol/L PARPi olaparib, 2.5 mmol/L RAD52i 6-hydroxy-DL-dopa and the indicated combinations. Results show mean % SD of colony numbers when compared with untreated cells (G) and mean % of tail DNA SD (H). I, HR activity in Lin-CD34+ cells from HR-proficient and HR-deficient AMLs (n = 3 of each). Results represent mean % SD of GFP+ cells in RFP+ cells. J, Lin-CD34+ cells from HR-proficient and HR-deficient AMLs (n = 3 of each) were treated with 25 mmol/L Polθi ART558, 1.25 mmol/L PARPi olaparib, 2.5 mmol/L RAD52i 6-hydroxy-DL–dopa and the indicated combinations. Results represent mean % SD of colonies in comparison with untreated cells. K, Survival curves of the AML mice treated with vehicle (C), Polθi RP6685, PARPi olaparib, and the combination. (B–D and F–J) Statistical significance when compared with: # another group, + control, and * corresponding individual treatments. K, ***, P < 0.001 in comparison to other groups.

    Techniques Used: Western Blot, Expressing, Activity Assay, Mutagenesis, Comparison, Control

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    Viability Assay:

    Article Title: Inhibitors of RAD52 recombination protein and methods using same
    Article Snippet: After two weeks, the colonies were fixed/stained with 0.05% of 10 mg/ml ethidium bromide in 50% ethanol and visualized with Alphaimager gel imager (Alpha Innotech). .. CML Viability Assay Lin-CD34+ primary CML and normal cells were obtained by magnetic sorting using the EasySep negative selection human progenitor cell enrichment cocktail followed by treatment with human CD34 positive selection cocktail (StemCell Technologies), and were subsequently cultured in StemSpan H3000 media (StemCell Technologies) supplemented with a cocktail of growth factors (100 ng/ml stem cell factor, 20 ng/ml interleukin3 [IL-3], 100 ng/ml fms-related tyrosine kinase 3 ligand, 20 ng/ml granulocyte colony-stimulating factor, 20 ng/ml IL-6). ..

    Article Title: Inhibitors of RAD52 recombination protein and methods using same
    Article Snippet: After two weeks, the colonies were fixed/stained with 0.05% of 10 mg/ml ethidium bromide in 50% ethanol and visualized with Alphaimager gel imager (Alpha Innotech). .. CML Viability Assay Lin-CD34+ primary CML and normal cells were obtained by magnetic sorting using the EasySep negative selection human progenitor cell enrichment cocktail followed by treatment with human CD34 positive selection cocktail (StemCell Technologies), and were subsequently cultured in StemSpan H3000 media (StemCell Technologies) supplemented with a cocktail of growth factors (100 ng/ml stem cell factor, 20 ng/ml interleukin3 [IL-3], 100 ng/ml fins-related tyrosine kinase 3 ligand, 20 ng/ml granulocyte colony-stimulating factor, 20 ng/ml IL-6). ..

    Selection:

    Article Title: Inhibitors of RAD52 recombination protein and methods using same
    Article Snippet: After two weeks, the colonies were fixed/stained with 0.05% of 10 mg/ml ethidium bromide in 50% ethanol and visualized with Alphaimager gel imager (Alpha Innotech). .. CML Viability Assay Lin-CD34+ primary CML and normal cells were obtained by magnetic sorting using the EasySep negative selection human progenitor cell enrichment cocktail followed by treatment with human CD34 positive selection cocktail (StemCell Technologies), and were subsequently cultured in StemSpan H3000 media (StemCell Technologies) supplemented with a cocktail of growth factors (100 ng/ml stem cell factor, 20 ng/ml interleukin3 [IL-3], 100 ng/ml fms-related tyrosine kinase 3 ligand, 20 ng/ml granulocyte colony-stimulating factor, 20 ng/ml IL-6). ..

    Article Title: Non-NAD-like PARP1 inhibitor enhanced synthetic lethal effect of NAD-like PARP inhibitors against BRCA1-deficient leukemia
    Article Snippet: Here we tested the hypothesis that combinations of two structurally different PARP1 inhibitors, NAD-like olaparib or talazoparib and non-NAD-like 5F02 ( ) result in more abundant elimination of BRCA1-deficient leukemia cells and reduced toxicity to normal counterparts. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1. caption a7 caption a8 5F02 enhanced the effect of olaparib against BRCA1-deficient CML cells. ( A ) Scheme illustrating anti-PARP1 activity of NAD-like (olaparib and talazoparib, both from Selleckchem) and non-NAD-like (5F02, synthesized as described in Supplemental Methods) inhibitors. ( B ) BCR-ABL1 –positive CML primary samples (n=3) and samples of normal hematopoietic cells (n=3) were characterized before [ 6 ]. .. Lin - CD34 + cells were obtained from mononuclear fractions by magnetic sorting using the EasySep negative selection human progenitor cell enrichment cocktail followed by human CD34 positive selection cocktail (StemCell Technologies) as described before [ 6 ]. .. Cells were incubated for 72 hrs with the indicated concentrations of olaparib (triangles), 5F02 (circles) and olaparib + 5F02 (squares) in IMDM supplemented with 10% FBS and growth factors (100 ng/ml SCF, 20 ng/ml IL-3, 100 ng/ml Flt-3 ligand, 20 ng/ml G-CSF, 20 ng/ml IL-6) followed by plating in Methocult (Stemcell Technologies).

    Article Title: Inhibition of the mutated c-KIT kinase in AML1-ETO–positive leukemia cells restores sensitivity to PARP inhibitor
    Article Snippet: Samples of normal hematopoietic cells were purchased from STEMCELL Technologies (Vancouver, BC, Canada). .. Lin − CD34 + cells were obtained from mononuclear fractions by magnetic sorting using EasySep negative selection human progenitor cell enrichment cocktail, followed by human CD34 positive selection cocktail (STEMCELL Technologies), as described previously. .. 12 The Kasumi-1 AML cell line harboring AML1-ETO + c-KIT(N822K) was purchased from the American Type Culture Collection.

    Article Title: Ruxolitinib-induced defects in DNA repair cause sensitivity to PARP inhibitors in myeloproliferative neoplasms
    Article Snippet: Samples of normal hematopoietic cells were purchased from Cambrex Bio Science (Walkersville, MD). .. Lin − CD34 + cells were obtained from mononuclear fractions by magnetic sorting using the EasySep negative selection human progenitor cell enrichment cocktail followed by the human CD34 + selection cocktail (StemCell Technologies) as described previously. ..

    Article Title: Inhibitors of RAD52 recombination protein and methods using same
    Article Snippet: After two weeks, the colonies were fixed/stained with 0.05% of 10 mg/ml ethidium bromide in 50% ethanol and visualized with Alphaimager gel imager (Alpha Innotech). .. CML Viability Assay Lin-CD34+ primary CML and normal cells were obtained by magnetic sorting using the EasySep negative selection human progenitor cell enrichment cocktail followed by treatment with human CD34 positive selection cocktail (StemCell Technologies), and were subsequently cultured in StemSpan H3000 media (StemCell Technologies) supplemented with a cocktail of growth factors (100 ng/ml stem cell factor, 20 ng/ml interleukin3 [IL-3], 100 ng/ml fins-related tyrosine kinase 3 ligand, 20 ng/ml granulocyte colony-stimulating factor, 20 ng/ml IL-6). ..

    Article Title: Simultaneous Targeting of PARP1 and RAD52 Triggers Dual Synthetic Lethality in BRCA-Deficient Tumor Cells
    Article Snippet: Samples of normal hematopoietic cells were purchased from Cambrex Bio Science. .. Lin − CD34 + cells were obtained from mononuclear fractions by magnetic sorting using the EasySep negative selection human progenitor cell enrichment cocktail followed by human CD34-positive selection cocktail (StemCell Technologies) as described previously ( ). ..

    Article Title: Poly(ADP-ribose) Polymerase 1, PARP1, modifies EZH2 and inhibits EZH2 histone methyltransferase activity after DNA damage
    Article Snippet: Normal hematopoietic cells were purchased from Cambrex Bio Science (Walkersville, MD, USA). .. Lin − CD34 + cells were obtained from mononuclear fractions by magnetic sorting using the EasySep negative selection human progenitor cell enrichment cocktail followed by the human CD34 positive selection cocktail (StemCell Technologies) as previously described [ , ]. .. Cells were incubated with olaparib, UNC1999 and/or daunorubicin (DNR) (all from Selleckchem) in IMDM supplemented with 10% FBS and growth factors (100 ng/μl SCF, 20 ng/μl IL-3, 100 ng/μl Flt-3 ligand, 20 ng/μl G-CSF, 20 ng/μl IL-6).

    Article Title: Tyrosine kinase inhibitor–induced defects in DNA repair sensitize FLT3(ITD)-positive leukemia cells to PARP1 inhibitors
    Article Snippet: Samples of normal hematopoietic cells were purchased from Cambrex Bio Science (Walkersville, MD). .. Lin − CD34 + cells were obtained from mononuclear fractions by magnetic sorting using EasySep negative selection human progenitor cell enrichment cocktail, followed by human CD34 positive selection cocktail (STEMCELL Technologies, Vancouver, BC, Canada), as described previously. .. 31 Primary murine cells Evi1-GFP–transgenic mice (GFP knockin to Evi1 locus does not affect the function of Evi1) were generously provided by Mineo Kurokawa (University of Tokyo), 32 and FLT3(ITD) -knockin mice 33 were obtained from The Jackson Laboratory.

    Cell Culture:

    Article Title: Inhibitors of RAD52 recombination protein and methods using same
    Article Snippet: After two weeks, the colonies were fixed/stained with 0.05% of 10 mg/ml ethidium bromide in 50% ethanol and visualized with Alphaimager gel imager (Alpha Innotech). .. CML Viability Assay Lin-CD34+ primary CML and normal cells were obtained by magnetic sorting using the EasySep negative selection human progenitor cell enrichment cocktail followed by treatment with human CD34 positive selection cocktail (StemCell Technologies), and were subsequently cultured in StemSpan H3000 media (StemCell Technologies) supplemented with a cocktail of growth factors (100 ng/ml stem cell factor, 20 ng/ml interleukin3 [IL-3], 100 ng/ml fms-related tyrosine kinase 3 ligand, 20 ng/ml granulocyte colony-stimulating factor, 20 ng/ml IL-6). ..

    Article Title: Inhibitors of RAD52 recombination protein and methods using same
    Article Snippet: After two weeks, the colonies were fixed/stained with 0.05% of 10 mg/ml ethidium bromide in 50% ethanol and visualized with Alphaimager gel imager (Alpha Innotech). .. CML Viability Assay Lin-CD34+ primary CML and normal cells were obtained by magnetic sorting using the EasySep negative selection human progenitor cell enrichment cocktail followed by treatment with human CD34 positive selection cocktail (StemCell Technologies), and were subsequently cultured in StemSpan H3000 media (StemCell Technologies) supplemented with a cocktail of growth factors (100 ng/ml stem cell factor, 20 ng/ml interleukin3 [IL-3], 100 ng/ml fins-related tyrosine kinase 3 ligand, 20 ng/ml granulocyte colony-stimulating factor, 20 ng/ml IL-6). ..



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    STEMCELL Technologies Inc easysep negative selection human progenitor cell enrichment cocktail (stemcell technologies)
    Targeting Polθ + PARP and Polθ + RAD52 induced dual synthetic lethality against HR-deficient leukemia cells. A, Western blot analysis showing the expression of indicated proteins in the nuclear fractions of Nalm6(RAD54+/+) and Nalm6(RAD54−/−) isogenic cell lines. B, HR activity in Nalm6(RAD54+/+) and Nalm6(RAD54−/−) cells: results represent mean % SD of GFP+ cells in RFP+ cells. Nalm6(RAD54+/+) and Nalm6(RAD54−/−) cells were treated for 72 hours (C) and 12 hours (D) with 25 mmol/L Polθi ART558, 2.5 nmol/L PARPi talazoparib, 10 mmol/L RAD52i 6-hydroxy-DL-dopa and the indicated combinations. Results show mean number SD of colony numbers (C) and mean % of tail DNA SD (D). E, Western blot analysis showing the expression of indicated proteins in the nuclear and cytosolic fractions of HEL cells with tet-inducible IDH1wt or IDH1(R132H) mutant. F, HR activity in HEL cells expressing IDH1wt or IDH1(R132H) mutant: results represent mean % SD of GFP+ cells in RFP+ cells. HEL cells expressing IDH1wt or IDH1(R132H) mutant were treated for 72 hours (G) and 12 hours (H) with 25 mmol/L Polθi ART558, 1 mmol/L PARPi olaparib, 2.5 mmol/L RAD52i 6-hydroxy-DL-dopa and the indicated combinations. Results show mean % SD of colony numbers when compared with untreated cells (G) and mean % of tail DNA SD (H). I, HR activity in <t>Lin-CD34+</t> cells from HR-proficient and HR-deficient AMLs (n = 3 of each). Results represent mean % SD of GFP+ cells in RFP+ cells. J, <t>Lin-CD34+</t> cells from HR-proficient and HR-deficient AMLs (n = 3 of each) were treated with 25 mmol/L Polθi ART558, 1.25 mmol/L PARPi olaparib, 2.5 mmol/L RAD52i 6-hydroxy-DL–dopa and the indicated combinations. Results represent mean % SD of colonies in comparison with untreated cells. K, Survival curves of the AML mice treated with vehicle (C), Polθi RP6685, PARPi olaparib, and the combination. (B–D and F–J) Statistical significance when compared with: # another group, + control, and * corresponding individual treatments. K, ***, P < 0.001 in comparison to other groups.
    Easysep Negative Selection Human Progenitor Cell Enrichment Cocktail (Stemcell Technologies), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+negative+selection+human+progenitor+cell+enrichment+cocktail+(stemcell+technologies)/easysep+negative+selection+human+progenitor+cell+enrichment+cocktail/pmc10654933-64-25-30
    Average 90 stars, based on 1 article reviews
    easysep negative selection human progenitor cell enrichment cocktail (stemcell technologies) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    Targeting Polθ + PARP and Polθ + RAD52 induced dual synthetic lethality against HR-deficient leukemia cells. A, Western blot analysis showing the expression of indicated proteins in the nuclear fractions of Nalm6(RAD54+/+) and Nalm6(RAD54−/−) isogenic cell lines. B, HR activity in Nalm6(RAD54+/+) and Nalm6(RAD54−/−) cells: results represent mean % SD of GFP+ cells in RFP+ cells. Nalm6(RAD54+/+) and Nalm6(RAD54−/−) cells were treated for 72 hours (C) and 12 hours (D) with 25 mmol/L Polθi ART558, 2.5 nmol/L PARPi talazoparib, 10 mmol/L RAD52i 6-hydroxy-DL-dopa and the indicated combinations. Results show mean number SD of colony numbers (C) and mean % of tail DNA SD (D). E, Western blot analysis showing the expression of indicated proteins in the nuclear and cytosolic fractions of HEL cells with tet-inducible IDH1wt or IDH1(R132H) mutant. F, HR activity in HEL cells expressing IDH1wt or IDH1(R132H) mutant: results represent mean % SD of GFP+ cells in RFP+ cells. HEL cells expressing IDH1wt or IDH1(R132H) mutant were treated for 72 hours (G) and 12 hours (H) with 25 mmol/L Polθi ART558, 1 mmol/L PARPi olaparib, 2.5 mmol/L RAD52i 6-hydroxy-DL-dopa and the indicated combinations. Results show mean % SD of colony numbers when compared with untreated cells (G) and mean % of tail DNA SD (H). I, HR activity in Lin-CD34+ cells from HR-proficient and HR-deficient AMLs (n = 3 of each). Results represent mean % SD of GFP+ cells in RFP+ cells. J, Lin-CD34+ cells from HR-proficient and HR-deficient AMLs (n = 3 of each) were treated with 25 mmol/L Polθi ART558, 1.25 mmol/L PARPi olaparib, 2.5 mmol/L RAD52i 6-hydroxy-DL–dopa and the indicated combinations. Results represent mean % SD of colonies in comparison with untreated cells. K, Survival curves of the AML mice treated with vehicle (C), Polθi RP6685, PARPi olaparib, and the combination. (B–D and F–J) Statistical significance when compared with: # another group, + control, and * corresponding individual treatments. K, ***, P < 0.001 in comparison to other groups.

    Journal: Molecular cancer research : MCR

    Article Title: Simultaneous Targeting of DNA Polymerase Theta and PARP1 or RAD52 Triggers Dual Synthetic Lethality in Homologous Recombination–Deficient Leukemia Cells

    doi: 10.1158/1541-7786.MCR-22-1035

    Figure Lengend Snippet: Targeting Polθ + PARP and Polθ + RAD52 induced dual synthetic lethality against HR-deficient leukemia cells. A, Western blot analysis showing the expression of indicated proteins in the nuclear fractions of Nalm6(RAD54+/+) and Nalm6(RAD54−/−) isogenic cell lines. B, HR activity in Nalm6(RAD54+/+) and Nalm6(RAD54−/−) cells: results represent mean % SD of GFP+ cells in RFP+ cells. Nalm6(RAD54+/+) and Nalm6(RAD54−/−) cells were treated for 72 hours (C) and 12 hours (D) with 25 mmol/L Polθi ART558, 2.5 nmol/L PARPi talazoparib, 10 mmol/L RAD52i 6-hydroxy-DL-dopa and the indicated combinations. Results show mean number SD of colony numbers (C) and mean % of tail DNA SD (D). E, Western blot analysis showing the expression of indicated proteins in the nuclear and cytosolic fractions of HEL cells with tet-inducible IDH1wt or IDH1(R132H) mutant. F, HR activity in HEL cells expressing IDH1wt or IDH1(R132H) mutant: results represent mean % SD of GFP+ cells in RFP+ cells. HEL cells expressing IDH1wt or IDH1(R132H) mutant were treated for 72 hours (G) and 12 hours (H) with 25 mmol/L Polθi ART558, 1 mmol/L PARPi olaparib, 2.5 mmol/L RAD52i 6-hydroxy-DL-dopa and the indicated combinations. Results show mean % SD of colony numbers when compared with untreated cells (G) and mean % of tail DNA SD (H). I, HR activity in Lin-CD34+ cells from HR-proficient and HR-deficient AMLs (n = 3 of each). Results represent mean % SD of GFP+ cells in RFP+ cells. J, Lin-CD34+ cells from HR-proficient and HR-deficient AMLs (n = 3 of each) were treated with 25 mmol/L Polθi ART558, 1.25 mmol/L PARPi olaparib, 2.5 mmol/L RAD52i 6-hydroxy-DL–dopa and the indicated combinations. Results represent mean % SD of colonies in comparison with untreated cells. K, Survival curves of the AML mice treated with vehicle (C), Polθi RP6685, PARPi olaparib, and the combination. (B–D and F–J) Statistical significance when compared with: # another group, + control, and * corresponding individual treatments. K, ***, P < 0.001 in comparison to other groups.

    Article Snippet: Lin − CD34 + cells were obtained from mononuclear fractions by magnetic sorting using the EasySep negative selection human progenitor cell enrichment cocktail followed by human CD34 positive selection cocktail (Stemcell Technologies) as described before ( 3 ).

    Techniques: Western Blot, Expressing, Activity Assay, Mutagenesis, Comparison, Control